Transcription

Transcription: DNA to RNA
1) Initiation
Determing which bit of DNA will be copied to RNA

• Promoter sequence: (when transcription initiation ocurrs) where DNA is unwound and unzipped so RNA Copy can be made; lots of T's and A's at all promoter sites
• RNA Polymerase: Enzyme that copies one strand of DNA into an RNA single-stranded copy.

-sigma factor: subunit of RNA plymerase that binds to DNA promoter sequences
-DNA must be unwound before its transcribed
2)Elongation: RNA strand synthesized by pairing complementary RNA bases w/ DNA template strand and hooking the bases together with sugar-phosphate backbone
Ex: TATATACCCTACCCCACA
AUAUUAGGGAUGGGGUGU (complementary base pairs)