Review- Notes of DNA replication

I. Properties of DNA
a. supercoiled
b double stranded w/ antiparrellel strands (point in opposite directions)
- DNA sugar phosphate backbone holds bases
- A,T, G, C in the orders they are in
-A and T are held together by 2 hydrogen bonds
- G and C are held together by 3 hydrogen bonds
( A and T bonds will be the first broken)

c. semiconservative replication
- one of the strands is original DNA strand the others are replicated strands

II. Steps in DNA replication
a. OriC: where DNA replication begins on bacterial chromosomes
*bacterial circular chromosome is supercoiled for DNA replication to begin it must be unwound and unzipped = this happens at the OriC site
* most likely A and T's b/c sequence of bases that is the easiest to unzip and detach
- Enzymes bind to the Ori C
- DNA A protein that bind to DNA and brings in DNA B
- DNA B helicase- enzyme that uncoils the coils of DNA

b. Helicase uncoils supercoils around the Ori C site
- unzips DNA double strands
primase- enzyme that sunthesizes small swatch of RNA complementary to part of the Oric C sequence
- primase attaches to unzipped DNA, synthesizes RNA primer

c. DNA polymerase attaches to RNA primer, synthesizes DNA, (DNA polymerase enzyme brings in bases complimentary to bases on the original strand using sugar phosphate backbone
template strand- DNA strand being copied that DNA polymerase is matching bases to

*DNA polymerase can only move in a 5' to 3' directions

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