Microbiology Lab Notes 9/22

Can E.Coli Bacteria become resistant to T4 bacteriphages?

Day 1:
1) Make a plate of agar that has T4 bacteriophages on it
2) Pipet .2 mL phage suspension on the agar and spread it by hockey sticking
3) Pick a colony of E. Coli
4) inoculate 100 mL nutrient broth then streak same colony on plate with phages
Day 2:

5) Take 100 mL nutrient E. Coli broth from colony and dilute it 1:10^5
6) Use diluted brother to inoculate 100 test tubes so that each test tube has 5 mL sterile broth and 1/10 mL of diluted broth in it
7) And 1 flask with 500 mL of sterile broth and 10 mL of diluted broth

Day 3:
8) Plate (hockey stick) 0.1 mL samples of each test tube onto plates with T4 phages
9) and plate same # of .1 mL samples from flask onto plates with T4 phages

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