Microbiology Lab Notes

Day 1
1) Make a plate with T4 phages:

  • pipet .2mL of T4 phages onto agar plate. Then hockey-stick the plate to cover the whole surface.

2) Pick E. Coli colony:
Pick a colony of E. Coli to culture in 100 mL of nutrient broth
Then inoculate broth
Streak that same colony on to the plate with the T4 phages

Day 2
3. Dilute bacterial growth 1:10^5: do this by taking a drop of bacterial broth from the first bottle and put into a pure sterile bottle of broth (100mL)
4. Use the, new, diluted broth to inoculate 100 test tubes: .1mL diluted broth with E. Coli the test tubes should already have 5mL of sterile broth.
Then take 10mL of diluted broth (with E. Coli) and put it into a 500mL flask.

Day 3
5. plate .1mL samples out of each test tube onto plate with T4 phages and plate the same number of .1 mL samples from flask onto plates with T4 phages. I think I am corrected in saying that Robson will only have us do about ten of each (test tubes and broth from the flask).

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