Micro lab notes 9/22

day 1
1. make plate w/t4 phages
* pipit 0.2 ml
phage suspension on agar + hockey stick
2. pick E.coli colony
- inoculate 100ml nutrient broth
then- streak same colony on plate w/ phages
day 2
3. divulte bacterial broth 1:10^5
4. use diluted broth to inoculate 100 test tubes: 5 ml sterlie broth w/ o.1 ml diluted broth
and 1 flask of broth: 10ml of diluted broth and 500ml of sterile broth
day 3
5. plate ( hockey stick) o.1ml samples out of each test tube onto plates w/t4
and- plate same number of 0.1 ml samples from flask onto plates with t4 phages

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