Lecture Notes Sept 23

After revisiting our experiments, we discuss bacterial flagella and how they work. What is the connection between this and mitochondria? Tune in next time to find out.
My Notes
In lab yesterday, we grabbed a random colony and used that colony to inoculate a nutrient broth then we streaked back and forth on a plate of agar that had T4 bacteriophages on it
Did interpretive dances involving pili and how pili can be used for motility by bacteria is because they have adhesive proteins on the tips of the pili which are able to attach to surfaces and then when the pili contract, that pulls the pilli rapidly forward
Twitching motility by attaching to surfaces and retracting
Motility facilitated by bacterial flagella (Article)
Talked about peripheral and intergral membrane proteins
Peripheral proteins are stuck to the outside of proteins inside a cell membrane and integral membrane proteins are going to expand the entire width of the cell membrane
Also talked about last time peripheral membrane proteins with adhesives at the end of the pilli’s can then be used for the pili to attach to surfaces and then retract resulting in Twitching motility
a. Pilli-Jerky motion of bacteria with pili along surface; pili attach to surface, retract
b. Flagellar motility-swimming smoothly through liquid. Flagella analogous to motor on a submarine
Arrangements of flagella on bacteria
Polar and peritrichous
Polar-the bacteria have flagella located on one end or on the other end of the bacteria but not all over

Bacterium with dual polar flagella
Peritrichous-Flagella all over
Means covered in hair

Bacterial flagella are Not the Same as Eukaryotic flagella
Bacterial Flagella
Smaller than eukaryotic flagella
Rigid: act like propellars with motors to move bacterial cells
Made of protein flagellin: this protein forms filament that acts as propeller
Flagellin filament attached to “motor” made of venous integral membrane proteins
This whole set of proteins making the flagella move is called the basal body
Eukaryotic Flagella
Flexible: Move like snakes
What are Eukaryotic flagella made of? Tubuline and dinine interact with each other which results in the snake-like motion of these flagella
Basal body and hook
What’s the difference between the basal body of a gram negative flagellum and The basal body of a gram positive flagellum?
How the basal body and hook works: flagellin filament is attached to a hook which is made of separate proteins then the flagellin filament, hook is turned then to the basal body of flagellum which is going to use ATP and break down ATP into ADP and inorganic phosphate and use the energy from that reaction to spin the flagellum
Do bacteria have mitochondria?
No, because they are prokaryotic
They are not Losers!

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