Lab Notes

Lab Notes Oct 27, 2009

Biofilms:
- Made up of proteins, carbohydrates, lipids, forming a goey snot-like structure surrounding bacteria
- Biofilms are only made at high populations densities: Quorum sensing trait

- The bacteria contain the genes for making a biofilm on their chromosomes

- A receptor (which is always on) receives the signaling molecule in it telling the cell to begin making a biofilm

Bacterial species studied:

Pseudomonas aeruginosa
PAO1: has genes for the signaling molecule: homoserine lactone; receptor and biofilm
JP2: (mutant strain) has all the genes that PAO1 does except the gene for the signaling molecule (homoserine lactone)

Ramnolipids- floaty goo in the biofilm
Pyocyannine: purple protein: antibacterial against gm +

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