Gram Stain Mistake

This is the mistake I made on my gram stain:
On the first gram stain I did on M. Luteus. This is supposed to be a gram-positive bacteria. After looking under the microscope, my gram stain appeared to be more of a pink color than purple, a.k.a. gram-negative. I figured out the mistake I made. I wasn't careful enough when putting the alcohol on the slide. You could say I "over-bleached" my bacteria. I went back to the lab later on that afternoon and did the experiment two more times using M. Luteus bacteria. I was very careful when putting the alcohol on my slide and my results were my slide showing up under the microscope as Gram-Positive both times. Hopefully after reading this no one makes the mistake I did even though most of the labs for this week have already taken place. Also any input would be greatly appreciated!
Amy Blass

Unless otherwise stated, the content of this page is licensed under Creative Commons Attribution-ShareAlike 3.0 License