11/4 notes

iv) a) mutation rate: 1:10^10 bases copied is different from original
Bacterial genome sizes:
*E coli 5 million base-pairs
* S. aureus-~3 million base pairs
* Chlamydia trachomatis- ~1 million base pairs
* Mycoplasma genitalium-500,000 bps
* Microbes that can survive in independently have more genes (thus larger genomes w/ more bps) than microbes that need a host.(Extreme example: viruses) Viruses have very small genomes b/c have few gene
How many mutant bacteria are there in a colony of 1 million E. coli?
1x 10^9 E coli x 5x10^6 bps=5x10^15bps in colony/ 1x10^10= 500,000
b) Mismatch repair system
* Mismatched bases identified by proofreading enzymes: methylated side identified
* Endonuclease cuts single non-methylated DNA strandīƒ Degrades stretch btw. cuts.
* DNA polymerase synthesizes new matching strand

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